Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum.
J Sep Sci
; 37(1-2): 165-70, 2014 Jan.
Article
em En
| MEDLINE
| ID: mdl-24166848
ABSTRACT
This study presents an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum. A new solvent system composed of petroleum ether/ethyl acetate/water (421, v/v/v) was developed for the liquid-liquid extraction of the crude extract from R. tanguticum. As a result, emodin, aloe-emodin, physcion, and chrysophanol were greatly enriched in the organic layer. In addition, an efficient method was successfully established to separate and purify the above anthraquinones by high-speed counter-current chromatography and preparative HPLC. This study supplies a new alternative method for the rapid enrichment, separation, and purification of emodin, aloe-emodin, physcione, and chrysophanol.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rheum
/
Extratos Vegetais
/
Cromatografia Líquida de Alta Pressão
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Antraquinonas
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Distribuição Contracorrente
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Extração Líquido-Líquido
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2014
Tipo de documento:
Article