Recombinant production, crystallization and preliminary structural characterization of Schistosoma japonicum profilin.

ABSTRACT

Helminthic parasites of the genus Schistosoma contain a tegumental membrane, which is of crucial importance for modulation of the host immune response and parasite survival. The actin cytoskeleton plays an important role in the function of the tegument. Profilins are among the most important proteins regulating actin dynamics. Schistosoma japonicum possesses one profilin-like protein, which has been characterized as a potential vaccine candidate. Notably, profilins are highly immunogenic molecules in many organisms. Here, the profilin from S. japonicum was expressed, purified and crystallized. A native data set to 1.91 Šresolution and a single-wavelength anomalous diffraction (SAD) data set to a resolution of 2.2 Šwere collected. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 31.82, b = 52.17, c = 59.79 Šand a = 35.29, b = 52.15, c = 59.82 Å, respectively.