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Application of immunoperoxidase staining in the cytodiagnosis of herpes simplex keratitis.
Diagn Cytopathol ; 1(4): 317-21, 1985.
Article em En | MEDLINE | ID: mdl-2424687
ABSTRACT
In corneal scraping smears from 13 patients with clinically suspected herpes simplex keratitis (HSK), HSK is demonstrated by means of peroxidase-antiperoxidase (PAP) technique with antisera to herpes simplex virus (HSV) in Papanicolaou-destained cellular samples. The staining for HSV antigen was present in seven cases of corneal scraping smears with superficial keratitis (dendritic and geographic ulcers) while six cases of stromal keratitis (deep keratitis) failed to show HSV antigen except in one case. Specific antigen for HSV was predominantly present in the cytoplasm rather than in the nucleus. Immunoreactions were negative with HSV antisera in patients with other infections and in those in a normal control group. Using the PAP technique, detection of HSV antigen in corneal scraping smears was of great value in the diagnosis of HSK, especially in cases of superficial keratitis.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ceratite Dendrítica Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Revista: Diagn Cytopathol Assunto da revista: PATOLOGIA Ano de publicação: 1985 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ceratite Dendrítica Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Revista: Diagn Cytopathol Assunto da revista: PATOLOGIA Ano de publicação: 1985 Tipo de documento: Article