Evaluation of serum phosphorylated neurofilament subunit NF-H as a prognostic biomarker in dogs with thoracolumbar intervertebral disc herniation.
Vet Surg
; 43(3): 289-93, 2014 Mar.
Article
em En
| MEDLINE
| ID: mdl-24467275
ABSTRACT
OBJECTIVE:
To investigate whether pNF-H is a prognostic biomarker of spinal cord injury (SCI) in paraplegic dogs with thoracolumbar intervertebral disc herniation (IVDH). STUDYDESIGN:
Prospective, case-control clinical study ANIMALS Dogs (n = 60) with SCI from IVDH and 6 healthy dogs.METHODS:
Serum from 60 thoracolumbar IVDH dogs (Grade 4 22 dogs; Grade 5 38 dogs) collected 1-3 days after injury, and 6 control dogs, was analyzed using enzyme-linked immunosorbent assay (ELISA) against a phosphorylated form of the high-molecular-weight neurofilament subunit NF-H (pNF-H). Serum pNF-H levels were compared between different IVDH grades and their prognostic value was investigated.RESULTS:
pNF-H levels were significantly greater in Grade 5 than Grade 4 dogs. There were significant differences in pNF-H levels between dogs that regained voluntarily ambulation and those that did not. All 8 dogs that had high pNF-H levels 1-3 days after injury did not regain the ability to walk after surgery.CONCLUSIONS:
Serum pNF-H levels might be a biomarker for predicting prognosis of canine SCI.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Neurofilamentos
/
Doenças do Cão
/
Degeneração do Disco Intervertebral
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Vet Surg
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Japão