Protein-guided RNA dynamics during early ribosome assembly.
Nature
; 506(7488): 334-8, 2014 Feb 20.
Article
em En
| MEDLINE
| ID: mdl-24522531
ABSTRACT
The assembly of 30S ribosomes requires the precise addition of 20 proteins to the 16S ribosomal RNA. How early binding proteins change the ribosomal RNA structure so that later proteins may join the complex is poorly understood. Here we use single-molecule fluorescence resonance energy transfer (FRET) to observe real-time encounters between Escherichia coli ribosomal protein S4 and the 16S 5' domain RNA at an early stage of 30S assembly. Dynamic initial S4-RNA complexes pass through a stable non-native intermediate before converting to the native complex, showing that non-native structures can offer a low free-energy path to protein-RNA recognition. Three-colour FRET and molecular dynamics simulations reveal how S4 changes the frequency and direction of RNA helix motions, guiding a conformational switch that enforces the hierarchy of protein addition. These protein-guided dynamics offer an alternative explanation for induced fit in RNA-protein complexes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Ribossômicas
/
RNA Ribossômico 16S
/
Subunidades Ribossômicas Menores de Bactérias
/
Simulação de Dinâmica Molecular
Idioma:
En
Revista:
Nature
Ano de publicação:
2014
Tipo de documento:
Article