Nitric oxide from mononuclear cells may be involved in platelet responsiveness to aspirin.
Eur J Clin Invest
; 44(5): 463-9, 2014 May.
Article
em En
| MEDLINE
| ID: mdl-24571196
ABSTRACT
BACKGROUND:
Several mechanisms have been proposed to explain why some platelets have a reduced response to aspirin (ASA). Among them, it was reported an increased circulating level of vitamin-D-binding protein (DBP). In addition, nitric oxide (NO) released from mononuclear cells was involved in the antiplatelet effects of ASA. The aim was to analyse the relationship between platelet response to ASA and both NO generation and vitamin-D-binding protein content in mononuclear cells. MATERIALS ANDMETHODS:
Mononuclear cells were obtained from patients with stable coronary artery disease that were divided by a platelet functionality test (PFA-100) as ASA-sensitive (n=23) and ASA resistant (n=27).RESULTS:
Both the release of NO (determined by nitrite+nitrate concentration) and the expression of endothelial-type NO synthase (eNOS) were higher in mononuclear cells from ASA sensitive as compared with those from ASA-resistant patients. There was a positive correlation between either the release of NO and the expression of eNOS protein in mononuclear cells with the ability of ASA to inhibit platelet activity. DBP content in mononuclear cells was higher in ASA resistant than in ASA sensitive. The level of DBP content in mononuclear cells was negatively associated with the ability of ASA to inhibit platelets. However, in vitro experiments suggested that there was no association between DBP and NO production by mononuclear cells.CONCLUSIONS:
Mononuclear cells from patients with platelets with lower responsiveness to ASA showed a reduced ability to produce NO.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Inibidores da Agregação Plaquetária
/
Aspirina
/
Óxido Nítrico
Limite:
Aged
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Eur J Clin Invest
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Espanha