Genetic modification of mouse effector and helper T lymphocytes expressing a chimeric antigen receptor.
Methods Mol Biol
; 1139: 177-87, 2014.
Article
em En
| MEDLINE
| ID: mdl-24619680
ABSTRACT
Genetic modification of primary mouse T cells with chimeric antigen receptors (CAR) has emerged as an important tool for optimizing adoptive T cell immunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR(+) T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8(+) and CD4(+) T lymphocytes for in vitro and in vivo characterization.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Receptores de Antígenos
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Engenharia Genética
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Linfócitos T Auxiliares-Indutores
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Linfócitos T CD8-Positivos
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Austrália