Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing.

Clermont, Olivier; Christenson, Julia K; Daubié, Anne-Sophie; Gordon, David M; Denamur, Erick

Clermont, Olivier; Christenson, Julia K; Daubié, Anne-Sophie; Gordon, David M; Denamur, Erick.

Afiliação

Clermont O; INSERM, IAME, UMR 1137, F-75018 Paris, France; Univ Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, F-75018 Paris, France.
Christenson JK; Division of Evolution, Ecology & Genetics, Research School of Biology, The Australian National University, Canberra ACT 0200, Australia.
Daubié AS; INSERM, IAME, UMR 1137, F-75018 Paris, France; Univ Paris Nord, IAME, UMR 1137, Sorbonne Paris Cité, F-75018 Paris, France.
Gordon DM; Division of Evolution, Ecology & Genetics, Research School of Biology, The Australian National University, Canberra ACT 0200, Australia.
Denamur E; INSERM, IAME, UMR 1137, F-75018 Paris, France; Univ Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, F-75018 Paris, France. Electronic address: erick.denamur@inserm.fr.

J Microbiol Methods ; 101: 24-7, 2014 Jun.

Article em En | MEDLINE | ID: mdl-24685601

RESUMO

We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.

Assuntos

Escherichia coli/genética; Genes Bacterianos/genética; Tipagem de Sequências Multilocus/métodos; Reação em Cadeia da Polimerase/métodos; Alelos; Animais; DNA Bacteriano/análise; DNA Bacteriano/genética; Infecções por Escherichia coli/microbiologia; Humanos

Palavras-chave

Allele-specific PCR; B2 phylogroup; B2 sub-group; Escherichia coli; Sequence type complex

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase / Escherichia coli / Tipagem de Sequências Multilocus / Genes Bacterianos Limite: Animals / Humans Idioma: En Revista: J Microbiol Methods Ano de publicação: 2014 Tipo de documento: Article País de afiliação: França

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