Isolation and antitumor activities of acidic polysaccharide from Gynostemma pentaphyllum Makino.
Carbohydr Polym
; 89(3): 942-7, 2012 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-24750884
ABSTRACT
Two acidic polysaccharides (GP-B1 and GP-C1) were obtained from Gynostemma pentaphyllum. The molecular weights (Mw) of the two fractions were 79 kDa for GP-B1 and 126 kDa for GP-C1. GP-B1 was composed of Gal, Ara, Man, Rha, Xyl, Glc, GalA and GlcA in a molar ration of 3.53.20.60.90.30.50.60.4. GP-C1 consisted of Gal, Ara, Man, Rha, Glc, and GlcA in the proportions of 2.11.00.30.50.40.9. Among them, GP-B1 treatment had a significant inhibitory effect on the growth of melanoma B16 in vivo and in vitro. Meanwhile GP-B1 could increase the relative spleen weight and stimulate the splenocyte proliferation alone or combined with ConA. Moreover, GP-B1 treatment induced an evident increase in the level of serum TNF-α, IFN-γ, and IL-12 and a reduction for IL-10 production. These results indicate that the antitumor effects of GP-B1 are associated with immunostimulation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Extratos Vegetais
/
Gynostemma
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China