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Whole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer.
Lohr, Jens G; Adalsteinsson, Viktor A; Cibulskis, Kristian; Choudhury, Atish D; Rosenberg, Mara; Cruz-Gordillo, Peter; Francis, Joshua M; Zhang, Cheng-Zhong; Shalek, Alex K; Satija, Rahul; Trombetta, John J; Lu, Diana; Tallapragada, Naren; Tahirova, Narmin; Kim, Sora; Blumenstiel, Brendan; Sougnez, Carrie; Lowe, Alarice; Wong, Bang; Auclair, Daniel; Van Allen, Eliezer M; Nakabayashi, Mari; Lis, Rosina T; Lee, Gwo-Shu M; Li, Tiantian; Chabot, Matthew S; Ly, Amy; Taplin, Mary-Ellen; Clancy, Thomas E; Loda, Massimo; Regev, Aviv; Meyerson, Matthew; Hahn, William C; Kantoff, Philip W; Golub, Todd R; Getz, Gad; Boehm, Jesse S; Love, J Christopher.
Afiliação
  • Lohr JG; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Dana-Farber Cancer Institute, Boston, Massachusetts, USA. [3] Harvard Medical School, Boston, Massachusetts, USA. [4].
  • Adalsteinsson VA; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [3].
  • Cibulskis K; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2].
  • Choudhury AD; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Dana-Farber Cancer Institute, Boston, Massachusetts, USA. [3] Harvard Medical School, Boston, Massachusetts, USA.
  • Rosenberg M; The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Cruz-Gordillo P; The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Francis JM; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
  • Zhang CZ; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
  • Shalek AK; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.
  • Satija R; The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Trombetta JJ; The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Lu D; The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Tallapragada N; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • Tahirova N; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • Kim S; The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Blumenstiel B; The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Sougnez C; The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Lowe A; Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Wong B; The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Auclair D; The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Van Allen EM; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Dana-Farber Cancer Institute, Boston, Massachusetts, USA. [3] Harvard Medical School, Boston, Massachusetts, USA.
  • Nakabayashi M; 1] Dana-Farber Cancer Institute, Boston, Massachusetts, USA. [2] Harvard Medical School, Boston, Massachusetts, USA.
  • Lis RT; Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
  • Lee GS; Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
  • Li T; Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
  • Chabot MS; Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
  • Ly A; Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Taplin ME; 1] Dana-Farber Cancer Institute, Boston, Massachusetts, USA. [2] Harvard Medical School, Boston, Massachusetts, USA.
  • Clancy TE; 1] Dana-Farber Cancer Institute, Boston, Massachusetts, USA. [2] Harvard Medical School, Boston, Massachusetts, USA. [3] Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Loda M; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Dana-Farber Cancer Institute, Boston, Massachusetts, USA. [3] Harvard Medical School, Boston, Massachusetts, USA. [4] Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Regev A; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [3] Howard Hughes Medical Institute, Chevy Chase, Maryland, USA.
  • Meyerson M; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Dana-Farber Cancer Institute, Boston, Massachusetts, USA. [3] Harvard Medical School, Boston, Massachusetts, USA.
  • Hahn WC; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Dana-Farber Cancer Institute, Boston, Massachusetts, USA. [3] Harvard Medical School, Boston, Massachusetts, USA. [4] Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Kantoff PW; 1] Dana-Farber Cancer Institute, Boston, Massachusetts, USA. [2] Harvard Medical School, Boston, Massachusetts, USA.
  • Golub TR; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Dana-Farber Cancer Institute, Boston, Massachusetts, USA. [3] Harvard Medical School, Boston, Massachusetts, USA. [4] Howard Hughes Medical Institute, Chevy Chase, Maryland, USA.
  • Getz G; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Boehm JS; The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Love JC; 1] The Eli and Edythe L. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [3] Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA.
Nat Biotechnol ; 32(5): 479-84, 2014 May.
Article em En | MEDLINE | ID: mdl-24752078
ABSTRACT
Comprehensive analyses of cancer genomes promise to inform prognoses and precise cancer treatments. A major barrier, however, is inaccessibility of metastatic tissue. A potential solution is to characterize circulating tumor cells (CTCs), but this requires overcoming the challenges of isolating rare cells and sequencing low-input material. Here we report an integrated process to isolate, qualify and sequence whole exomes of CTCs with high fidelity using a census-based sequencing strategy. Power calculations suggest that mapping of >99.995% of the standard exome is possible in CTCs. We validated our process in two patients with prostate cancer, including one for whom we sequenced CTCs, a lymph node metastasis and nine cores of the primary tumor. Fifty-one of 73 CTC mutations (70%) were present in matched tissue. Moreover, we identified 10 early trunk and 56 metastatic trunk mutations in the non-CTC tumor samples and found 90% and 73% of these mutations, respectively, in CTC exomes. This study establishes a foundation for CTC genomics in the clinic.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Próstata / Exoma / Células Neoplásicas Circulantes Limite: Humans / Male Idioma: En Revista: Nat Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2014 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Próstata / Exoma / Células Neoplásicas Circulantes Limite: Humans / Male Idioma: En Revista: Nat Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2014 Tipo de documento: Article