One role of hydration water in proteins: key to the "softening" of short time intraprotein collective vibrations of a specific length scale.
Soft Matter
; 10(24): 4298-303, 2014 Jun 28.
Article
em En
| MEDLINE
| ID: mdl-24789017
ABSTRACT
High resolution inelastic X-ray scattering (IXS) experiments show that the "phonon energy softening" and "phonon population enhancement" observed in a hydrated native protein when increasing the temperature from 200 K to physiological temperature are not directly related to the protein structure. Such phenomena were also observed in a denatured sample without a defined tertiary structure and with a limited residual secondary structure. However, in a dry sample, such "softening" is strongly suppressed. These facts suggest that the above-mentioned protein "softening" phenomenon is water-induced. In addition, increasing the hydration level can also induce "phonon energy softening" at room temperature, but not at 200 K. This change may be due to a qualitative difference in the dynamics of hydration water at 200 K and at room temperature.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Água
/
Muramidase
/
Quimotripsinogênio
/
Fônons
Tipo de estudo:
Qualitative_research
Idioma:
En
Revista:
Soft Matter
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos