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The cloning and expression in Escherichia coli of sequences coding for p24, the core protein of human immunodeficiency virus, and the use of the recombinant protein in characterizing a panel of monoclonal antibodies against the viral p24 protein.
Spence, R P; Jarvill, W M; Ferns, R B; Tedder, R S; Parker, D.
Afiliação
  • Spence RP; Department of Molecular Biology, Wellcome Biotech, Beckenham, Kent, U.K.
J Gen Virol ; 70 ( Pt 11): 2843-51, 1989 Nov.
Article em En | MEDLINE | ID: mdl-2479710
The sequences encoding the p24 core protein of human immunodeficiency virus type 1 were identified in a cDNA library made from infected CEM cells. The nucleotide sequence of the DNA coding for p24 was shown to be very similar but not identical to the sequences of lymphadenopathy virus and human T-cell leukaemia virus type IIIb. These sequences were expressed in Escherichia coli at the amino terminus of beta-galactosidase and used to screen a panel of monoclonal antibodies raised against virus-expressed p24. Regions containing the epitopes of five of the monoclonal antibodies were located using a series of amino- and carboxy-terminal deletion mutants of the recombinant p24 protein.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígenos HIV / Produtos do Gene gag / Proteínas do Core Viral / HIV Idioma: En Revista: J Gen Virol Ano de publicação: 1989 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígenos HIV / Produtos do Gene gag / Proteínas do Core Viral / HIV Idioma: En Revista: J Gen Virol Ano de publicação: 1989 Tipo de documento: Article