The Arabidopsis LecRK-VI.2 associates with the pattern-recognition receptor FLS2 and primes Nicotiana benthamiana pattern-triggered immunity.
Plant J
; 79(2): 243-55, 2014 Jul.
Article
em En
| MEDLINE
| ID: mdl-24844677
ABSTRACT
Pattern-triggered immunity (PTI) is broad spectrum and manipulation of PTI is believed to represent an attractive way to engineer plants with broad-spectrum disease resistance. PTI is activated upon perception of microbe-associated molecular patterns (MAMPs) by pattern-recognition receptors (PRRs). We have recently demonstrated that the L-type lectin receptor kinase-VI.2 (LecRK-VI.2) positively regulates Arabidopsis thaliana PTI. Here we show through in vitro pull-down, bimolecular fluorescence complementation and co-immunoprecipitation analyses that LecRK-VI.2 associates with the PRR FLS2. We also demonstrated that LecRK-VI.2 from the cruciferous plant Arabidopsis remains functional after interfamily transfer to the Solanaceous plant Nicotiana benthamiana. Wild tobacco plants ectopically expressing LecRK-VI.2 were indeed more resistant to virulent hemi-biotrophic and necrotrophic bacteria, but not to the fungal pathogen Botrytis cinerea suggesting that, as with Arabidopsis, the LecRK-VI.2 protective effect in N. benthamiana is bacteria specific. Ectopic expression of LecRK-VI.2 in N. benthamiana primed PTI-mediated reactive oxygen species production, mitogen-activated protein kinase (MAPK) activity, callose deposition and gene expression upon treatment with the MAMP flagellin. Our findings identified LecRK-VI.2 as a member of the FLS2 receptor complex and suggest that heterologous expression of components of PRR complexes can be used as tools to engineer plant disease resistance to bacteria.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nicotiana
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Arabidopsis
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Proteínas de Arabidopsis
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Imunidade Vegetal
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Imunidade Inata
Tipo de estudo:
Prognostic_studies
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Risk_factors_studies
Idioma:
En
Revista:
Plant J
Assunto da revista:
BIOLOGIA MOLECULAR
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BOTANICA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Taiwan