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AB5075, a Highly Virulent Isolate of Acinetobacter baumannii, as a Model Strain for the Evaluation of Pathogenesis and Antimicrobial Treatments.
Jacobs, Anna C; Thompson, Mitchell G; Black, Chad C; Kessler, Jennifer L; Clark, Lily P; McQueary, Christin N; Gancz, Hanan Y; Corey, Brendan W; Moon, Jay K; Si, Yuanzheng; Owen, Matthew T; Hallock, Justin D; Kwak, Yoon I; Summers, Amy; Li, Charles Z; Rasko, David A; Penwell, William F; Honnold, Cary L; Wise, Matthew C; Waterman, Paige E; Lesho, Emil P; Stewart, Rena L; Actis, Luis A; Palys, Thomas J; Craft, David W; Zurawski, Daniel V.
Afiliação
  • Jacobs AC; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Thompson MG; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Black CC; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Kessler JL; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Clark LP; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • McQueary CN; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Gancz HY; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Corey BW; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Moon JK; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Si Y; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Owen MT; Division of Orthopaedics, University of Alabama at Birmingham, Birmingham, Alabama, USA.
  • Hallock JD; Division of Orthopaedics, University of Alabama at Birmingham, Birmingham, Alabama, USA.
  • Kwak YI; Multidrug-resistant Organism and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Summers A; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Li CZ; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Rasko DA; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Penwell WF; Department of Microbiology, Miami University, Oxford, Ohio, USA.
  • Honnold CL; Department of Pathology, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Wise MC; Department of Pathology, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Waterman PE; Multidrug-resistant Organism and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Lesho EP; Multidrug-resistant Organism and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Stewart RL; Division of Orthopaedics, University of Alabama at Birmingham, Birmingham, Alabama, USA.
  • Actis LA; Department of Microbiology, Miami University, Oxford, Ohio, USA.
  • Palys TJ; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Craft DW; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
  • Zurawski DV; Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA daniel.v.zurawski.ctr@mail.mil.
mBio ; 5(3): e01076-14, 2014 May 27.
Article em En | MEDLINE | ID: mdl-24865555
ABSTRACT
UNLABELLED Acinetobacter baumannii is recognized as an emerging bacterial pathogen because of traits such as prolonged survival in a desiccated state, effective nosocomial transmission, and an inherent ability to acquire antibiotic resistance genes. A pressing need in the field of A. baumannii research is a suitable model strain that is representative of current clinical isolates, is highly virulent in established animal models, and can be genetically manipulated. To identify a suitable strain, a genetically diverse set of recent U.S. military clinical isolates was assessed. Pulsed-field gel electrophoresis and multiplex PCR determined the genetic diversity of 33 A. baumannii isolates. Subsequently, five representative isolates were tested in murine pulmonary and Galleria mellonella models of infection. Infections with one strain, AB5075, were considerably more severe in both animal models than those with other isolates, as there was a significant decrease in survival rates. AB5075 also caused osteomyelitis in a rat open fracture model, while another isolate did not. Additionally, a Tn5 transposon library was successfully generated in AB5075, and the insertion of exogenous genes into the AB5075 chromosome via Tn7 was completed, suggesting that this isolate may be genetically amenable for research purposes. Finally, proof-of-concept experiments with the antibiotic rifampin showed that this strain can be used in animal models to assess therapies under numerous parameters, including survival rates and lung bacterial burden. We propose that AB5075 can serve as a model strain for A. baumannii pathogenesis due to its relatively recent isolation, multidrug resistance, reproducible virulence in animal models, and genetic tractability. IMPORTANCE The incidence of A. baumannii infections has increased over the last decade, and unfortunately, so has antibiotic resistance in this bacterial species. A. baumannii is now responsible for more than 10% of all hospital-acquired infections in the United States and has a >50% mortality rate in patients with sepsis and pneumonia. Most research on the pathogenicity of A. baumannii focused on isolates that are not truly representative of current multidrug-resistant strains isolated from patients. After screening of a panel of isolates in different in vitro and in vivo assays, the strain AB5075 was selected as more suitable for research because of its antibiotic resistance profile and increased virulence in animal models. Moreover, AB5075 is susceptible to tetracycline and hygromycin, which makes it amenable to genetic manipulation. Taken together, these traits make AB5075 a good candidate for use in studying virulence and pathogenicity of this species and testing novel antimicrobials.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Acinetobacter / Acinetobacter baumannii Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Revista: MBio Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Acinetobacter / Acinetobacter baumannii Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Revista: MBio Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Estados Unidos