Broadening the scope of Baeyer-Villiger monooxygenase activities toward α,ß-unsaturated ketones: a promising route to chiral enol-lactones and ene-lactones.

ABSTRACT

Three regiodivergent Baeyer-Villiger mono-oxygenases (enantioselectively) oxidized a series of cyclic α,ß-unsaturated ketones into (chiral) either enol-lactones or ene-lactones. An easy-to-use and efficient biocatalytic process based on a host-microorganism deprived of unwanted activities (knock-out mutant) was developed to enable the exclusive synthesis of unsaturated lactones.