Ozone dosing alters the biological potential and therapeutic outcomes of plasma rich in growth factors.
J Periodontal Res
; 50(2): 240-7, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-24957247
ABSTRACT
BACKGROUND AND OBJECTIVE:
Until now, ozone has been used in a rather empirical way. This in-vitro study investigates, for the first time, whether different ozone treatments of plasma rich in growth factors (PRGF) alter the biological properties and outcomes of this autologous platelet-rich plasma. MATERIAL ANDMETHODS:
Human plasma rich in growth factors was treated with ozone using one of the following protocols a continuous-flow method; or a syringe method in which constant volumes of ozone and PRGF were mixed. In both cases, ozone was added before, during and after the addition of calcium chloride. Three ozone concentrations, of the therapeutic range 20, 40 and 80 µg/mL, were tested. Fibrin clot properties, growth factor content and the proliferative effect on primary osteoblasts and gingival fibroblasts were evaluated.RESULTS:
Ozone treatment of PRGF using the continuous flow protocol impaired formation of the fibrin scaffold, drastically reduced the levels of growth factors and significantly decreased the proliferative potential of PRGF on primary osteoblasts and gingival fibroblasts. In contrast, treatment of PRGF with ozone using the syringe method, before, during and after the coagulation process, did not alter the biological outcomes of the autologous therapy.CONCLUSION:
These findings suggest that ozone dose and the way that ozone combines with PRGF may alter the biological potential and therapeutic outcomes of PRGF.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ozônio
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Plasma Rico em Plaquetas
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Revista:
J Periodontal Res
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Espanha