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Differential Angiogenic Gene Expression in TP53 Wild-Type and Mutant Ovarian Cancer Cell Lines.
Davidson, Brittany Anne; Rubatt, Jennifer M; Corcoran, David L; Teoh, Deanna K; Bernardini, Marcus Q; Grace, Lisa A; Soper, William John; Berchuck, Andrew; Siamakpour-Reihani, Sharareh; Chen, Wei; Owzar, Kouros; Murphy, Susan K; Secord, Angeles Alvarez.
Afiliação
  • Davidson BA; Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Duke Cancer Institute , Durham, NC , USA.
  • Rubatt JM; Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Duke Cancer Institute , Durham, NC , USA.
  • Corcoran DL; Institute for Genome Sciences and Policy, Duke University Medical Center , Durham, NC , USA.
  • Teoh DK; Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Duke Cancer Institute , Durham, NC , USA.
  • Bernardini MQ; Gynecology Oncology, Toronto-Sunnybrook Regional Cancer Centre , Toronto, ON , Canada.
  • Grace LA; Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Duke Cancer Institute , Durham, NC , USA.
  • Soper WJ; Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Duke Cancer Institute , Durham, NC , USA.
  • Berchuck A; Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Duke Cancer Institute , Durham, NC , USA.
  • Siamakpour-Reihani S; Department of Radiation Oncology, Duke University Medical Center , Durham, NC , USA.
  • Chen W; Department of Biostatistics and Bioinformatics, Duke University Medical Center , Durham, NC , USA.
  • Owzar K; Department of Biostatistics and Bioinformatics, Duke University Medical Center , Durham, NC , USA.
  • Murphy SK; Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Duke Cancer Institute , Durham, NC , USA.
  • Secord AA; Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Duke Cancer Institute , Durham, NC , USA.
Front Oncol ; 4: 163, 2014.
Article em En | MEDLINE | ID: mdl-24999452
OBJECTIVES: Underlying mechanisms regulating angiogenesis in ovarian cancer have not been completely elucidated. Evidence suggests that the TP53 tumor suppressor pathway and tumor microenvironment play integral roles. We utilized microarray technology to study the interaction between TP53 mutational status and hypoxia on angiogenic gene expression. METHODS: Affymetrix U133A arrays were analyzed for angiogenic gene expression in 19 ovarian cancer cell lines stratified both by TP53 mutation status and A2780 wild-type (wt) TP53 vs. mutated (m) TP53 cell lines after treatment under hypoxic conditions or with ionizing radiation. RESULTS: Twenty-eight differentially expressed angiogenic genes were identified in the mTP53 cell lines compared to wtTP53 lines. Five genes were upregulated in mTP53 cells: 40% involved in extracellular matrix (ECM) degradation [matrix metalloproteinase 10 (MMP10)/15] and 60% in angiogenesis (fibroblast growth factor receptor 3/VEGFA/ephrin receptor-B4). Twenty-three genes were upregulated in wtTP53: nearly 22% were ECM constituents or involved in ECM degradation; over 40% were growth factors or mediators of angiogenesis. Five genes were upregulated in the A2780mTP53 cells: 40% involved in ECM remodeling (MMP10, ADAMTS1), 40% with pro-angiogenic activity (EFNB2, factor 2 receptor), and 20% with anti-angiogenic properties (ADAMTS1). Three genes were upregulated in hypoxia treated cells compared to controls: one with anti-angiogenic activity (angiopoietin-like 4) and two with pro-angiogenic activity (VEGFA, EFNA3). No significant gene fold changes were noted after exposure to radiation. Four genes continued to demonstrate significant differential expression (p ≤ 0.05) after adjusting for multiple comparisons. These genes included endoglin upregulation in wt lines (pro-angiogenesis) and upregulation of FGF20 (growth factor), ADAMTS1 (anti-angiogenesis) and MMP10 (ECM degradation) in mTP53 cell lines. CONCLUSION: Our exploratory findings indicate that non-overlapping angiogenic pathways may be altered by TP53 mutations and hypoxic conditions in the tumor microenvironment. Further evaluation is needed for confirmation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Front Oncol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Front Oncol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Estados Unidos