Rapid, sensitive and specific detection of OXA-48-like-producing Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
J Microbiol Methods
; 105: 88-91, 2014 Oct.
Article
em En
| MEDLINE
| ID: mdl-25016131
ABSTRACT
The emergence of Enterobacteriaceae producing the class D carbapenemase OXA-48 has become a public health concern, particularly in southern Europe and in the Mediterranean area. The existing MALDI-TOF MS assays that detect carbapenemase activity have disregarded the Enterobacteriaceae producing OXA-48-like enzymes. Here, we assessed the ability of an optimized MALDI-TOF MS imipenem hydrolysis assay to detect OXA-48-like-producing Enterobacteriaceae. We tested 372 non duplicate isolates of Enterobacteriaceae from 9 species, among which 92 harbored a blaOXA-48-like gene and 180 were non-susceptible to ertapenem. The vast majority (98.9%) of the OXA-48-like producers were detected and 97.8% of the ertapenem non-susceptible isolates were correctly classified. From the agar plate to the final result, the turnaround time of the test was 90min. This quick, easy and accurate MALDI-TOF MS test can expedite the implementation of hygiene measures to prevent dissemination of OXA-48-like-producing Enterobacteriaceae and limit outbreaks.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Beta-Lactamases
/
Imipenem
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Enterobacteriaceae
/
Antibacterianos
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
J Microbiol Methods
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
França