Pulmonary overexpression of inhibitor κBα decreases the severity of ventilator-induced lung injury in a rat model.
Br J Anaesth
; 113(6): 1046-54, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25053119
ABSTRACT
BACKGROUND:
Activation of the nuclear factor-κB (NF-κB) pathway is central to the pathogenesis of lung injury and inflammation. We determined whether targeted overexpression of inhibitor-κBα (IκBα) in the lung could decrease the severity of ventilator-induced lung injury (VILI).METHODS:
Anaesthetized adult male Sprague-Dawley rats were randomly allocated to undergo intratracheal instillation of (i) vehicle alone (surfactant, n=10); (ii) 1×10(10) adeno-associated virus encoding IκBα (AAV-IκBα, n=10); (iii) 5×10(10) AAV-IκBα (n=10); and (iv) 1×10(10) AAV-Null (n=5). This was followed by 4 h of injurious mechanical ventilation. Subsequent experiments examined the effect of IκBα overexpression in animals undergoing 'protective' mechanical ventilation.RESULTS:
IκBα overexpression increased survival duration at both the lower [3.8 h (0.4)] and higher [3.6 h (0.7)] doses compared with vehicle [2.7 h (1.0)] or the null transgene [2.2 h (0.8)]. IκBα overexpression reduced the alveolar-arterial oxygen gradient (kPa) at both the lower [53 (21)] and higher [52 (19)] doses compared with vehicle [75 (8.5)] or the null transgene [70 (15)], decreased alveolar neutrophil infiltration, and reduced alveolar concentrations of interleukin (IL)-1ß and IL-10. The lower IκBα dose was as effective as the higher dose. IκBα overexpression had no effect in the setting of protective lung ventilation.CONCLUSIONS:
Inhibition of pulmonary NF-κB activity by IκBα overexpression reduced the severity of VILI in a rat model.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Terapia Genética
/
Proteínas I-kappa B
/
Lesão Pulmonar Induzida por Ventilação Mecânica
/
Pulmão
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Br J Anaesth
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Irlanda