Property peculiarities of the atelocollagen-hyaluronan conjugates crosslinked with a short chain di-oxirane compound.
Mater Sci Eng C Mater Biol Appl
; 42: 243-53, 2014 Sep.
Article
em En
| MEDLINE
| ID: mdl-25063116
ABSTRACT
Minimal amounts of a short-chain bifunctional crosslinker of about 1.3 nm length, the 1,4-butanediol-diglycidyl ether (BDDGE), were used to generate atelocollagen-hyaluronan conjugates in hydrogel state. Two a priori constraints were considered in recipe/procedure developing (i) working in nondenaturing conditions, and (ii) ensuring a low cytotoxicity of the final product. Both atelocollagen (aK) and hyaluronan (NaHyal) were accurately purified to reduce their molecular-weight dispersity, in order to ensure the reproducibility of hydrogels characteristics. 15 aKNaHyal weight ratios and 12.5 to 15 α-NH2BDDGE molar ratios were found to be the most favorable recipe prescriptions that allow the obtaining of rheo-mechanically stable hydrogels, able to be manipulated during cell culturing protocols. Experiments revealed two unexpected effects due to the crosslinking reactions mediated by a short-chain molecule (i) the occurrence of two thresholds in the rheological behavior of the hydrogels, related with the amount of added crosslinker, and (ii) a quasi-denaturation side-effect induced over the protein component by large or in excess amounts of crosslinker.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Colágeno
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Hidrogel de Polietilenoglicol-Dimetacrilato
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Compostos de Epóxi
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Ácido Hialurônico
Idioma:
En
Revista:
Mater Sci Eng C Mater Biol Appl
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Romênia