Oxidative stress activates AMPK in cultured cells primarily by increasing cellular AMP and/or ADP.
FEBS Lett
; 588(18): 3361-6, 2014 Sep 17.
Article
em En
| MEDLINE
| ID: mdl-25084564
ABSTRACT
AMPK is known to be activated by oxidative stress. Addition of glucose oxidase to cells generates H2O2 at a constant rate that is opposed by enzymic degradation, providing a good model for physiological oxidative stress. AMPK activation by glucose oxidase correlated with increases in cellular AMPATP and was greatly reduced in cells expressing an AMP-insensitive AMPK mutant, although a small degree of activation remained. The effects of increased AMP were partly due to inhibition of Thr172 dephosphorylation. These results suggest that changes in adenine nucleotides, rather than direct oxidative modification, are the major drivers of AMPK activation during oxidative stress.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Difosfato de Adenosina
/
Monofosfato de Adenosina
/
Estresse Oxidativo
/
Proteínas Quinases Ativadas por AMP
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Reino Unido