[GC-MS analysis of chemical components of volatile oil from Viburnm schensianum leaf and their antibacterial activity].

ABSTRACT

OBJECTIVE: To analyze the chemical constituents of the volatile oil from Viburnm schensianum leaves and investigate their antibacterial activity in vitro. METHODS: The volatile oil from Viburnm schensianum leaves were extracted by steam distillation. The chemical compositions were separated and analyzed by GC-MS. Their relative percentage content were calculated with peak area normalization method. Agar dilution method was used to determine the minimum inhibitory concentration of the volatile oil and Ciprofloxacin to the five kinds of experimental strains. RESULTS: 54 compounds were isolated and 45 compounds were identified. The volatile oil showed strong antibacterial activity against Staphyloccocus aureus, and the minimum inhibitory concentration was 0. 032 mg/mL. CONCLUSION: The main chemical constituents of the volatile oil are n-hexadecanoic acid (29.24%), 4-methy-l--(1-methylethyl)-bicyclo [3.1.0] hex-3-en-2-one (5.71%), 3-methyl-4-isopropylphenol (5.68%)and linolenyl alcohol (4.84%). The volatile oil has strong antibacterial activity against Staphyloccocus aureus.