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Multiplexed aberration measurement for deep tissue imaging in vivo.
Wang, Chen; Liu, Rui; Milkie, Daniel E; Sun, Wenzhi; Tan, Zhongchao; Kerlin, Aaron; Chen, Tsai-Wen; Kim, Douglas S; Ji, Na.
Afiliação
  • Wang C; 1] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA. [2] State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai, China. [3].
  • Liu R; 1] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA. [2].
  • Milkie DE; 1] Coleman Technologies, Inc., Newtown Square, Pennsylvania, USA. [2].
  • Sun W; Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
  • Tan Z; Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
  • Kerlin A; Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
  • Chen TW; Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
  • Kim DS; Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
  • Ji N; Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
Nat Methods ; 11(10): 1037-40, 2014 Oct.
Article em En | MEDLINE | ID: mdl-25128976
ABSTRACT
We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Encéfalo / Óptica e Fotônica / Neuroimagem / Luz Limite: Animals Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2014 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Encéfalo / Óptica e Fotônica / Neuroimagem / Luz Limite: Animals Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2014 Tipo de documento: Article