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WRNIP1 functions upstream of DNA polymerase η in the UV-induced DNA damage response.
Yoshimura, Akari; Kobayashi, Yume; Tada, Shusuke; Seki, Masayuki; Enomoto, Takemi.
Afiliação
  • Yoshimura A; Molecular Cell Biology Laboratory, Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, Musashino University, 1-1-20 Shinmachi, Nishitokyo-shi, Tokyo 202-8585, Japan. Electronic address: akari_yo@stu.musashino-u.ac.jp.
  • Kobayashi Y; Molecular Cell Biology Laboratory, Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, Musashino University, 1-1-20 Shinmachi, Nishitokyo-shi, Tokyo 202-8585, Japan.
  • Tada S; Department of Medical Biochemistry, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi, Chiba 274-8510, Japan.
  • Seki M; Department of Biochemistry, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai-shi, Miyagi 981-8558, Japan.
  • Enomoto T; Molecular Cell Biology Laboratory, Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, Musashino University, 1-1-20 Shinmachi, Nishitokyo-shi, Tokyo 202-8585, Japan.
Biochem Biophys Res Commun ; 452(1): 48-52, 2014 Sep 12.
Article em En | MEDLINE | ID: mdl-25139235
WRNIP1 (WRN-interacting protein 1) was first identified as a factor that interacts with WRN, the protein that is defective in Werner syndrome (WS). WRNIP1 associates with DNA polymerase η (Polη), but the biological significance of this interaction remains unknown. In this study, we analyzed the functional interaction between WRNIP1 and Polη by generating knockouts of both genes in DT40 chicken cells. Disruption of WRNIP1 in Polη-disrupted (POLH(-/-)) cells suppressed the phenotypes associated with the loss of Polη: sensitivity to ultraviolet light (UV), delayed repair of cyclobutane pyrimidine dimers (CPD), elevated frequency of mutation, elevated levels of UV-induced sister chromatid exchange (SCE), and reduced rate of fork progression after UV irradiation. These results suggest that WRNIP1 functions upstream of Polη in the response to UV irradiation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Raios Ultravioleta / Dano ao DNA / DNA / Proteínas de Transporte / Proteínas de Ligação a DNA / DNA Polimerase Dirigida por DNA Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Raios Ultravioleta / Dano ao DNA / DNA / Proteínas de Transporte / Proteínas de Ligação a DNA / DNA Polimerase Dirigida por DNA Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2014 Tipo de documento: Article