Aging alters contractile properties and fiber morphology in pigeon skeletal muscle.
J Comp Physiol B
; 184(8): 1031-9, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25150060
ABSTRACT
In this study, we tested the hypothesis that skeletal muscle from pigeons would display age-related alterations in isometric force and contractile parameters as well as a shift of the single muscle fiber cross-sectional area (CSA) distribution toward smaller fiber sizes. Maximal force output, twitch contraction durations and the force-frequency relationship were determined in tensor propatagialis pars biceps muscle from young 3-year-old pigeons, middle-aged 18-year-old pigeons, and aged 30-year-old pigeons. The fiber CSA distribution was determined by planimetry from muscle sections stained with hematoxylin and eosin. Maximal force output of twitch and tetanic contractions was greatest in muscles from young pigeons, while the time to peak force of twitch contractions was longest in muscles from aged pigeons. There were no changes in the force-frequency relationship between the age groups. Interestingly, the fiber CSA distribution in aged muscles revealed a greater number of larger sized muscle fibers, which was verified visually in histological images. Middle-aged and aged muscles also displayed a greater amount of slow myosin containing muscle fibers. These data demonstrate that muscles from middle-aged and aged pigeons are susceptible to alterations in contractile properties that are consistent with aging, including lower force production and longer contraction durations. These functional changes were supported by the appearance of slow myosin containing muscle fibers in muscles from middle-aged and aged pigeons. Therefore, the pigeon may represent an appropriate animal model for the study of aging-related alterations in skeletal muscle function and structure.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Columbidae
/
Envelhecimento
/
Músculo Esquelético
/
Fibras Musculares Esqueléticas
/
Contração Muscular
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Comp Physiol B
Assunto da revista:
BIOQUIMICA
/
FISIOLOGIA
/
METABOLISMO
Ano de publicação:
2014
Tipo de documento:
Article