The changes of Proteome in MG-63 cells after induced by calcitonin gene-related peptide.

ABSTRACT

The aim of this study was to elucidate the changes of Proteomics in MG-63 cells after induced by calcitonin gene-related peptide (CGRP). The subcultured MG63 cells were randomly divided into CGRP group and the control group. Two-dimensional electrophoresis (2-DE), coomassie brilliant blue gel staining and mass spectrometry were used to analyze the changes of protein extracted from the two groups. Six protein spots with significant differences were selected to take in-gel digestion, peptide mass fingerprint analysis and IPI database search. There were more than 967±17 protein spots separated by Two-dimensional gel electrophoresis and the matching rate was (85.1±1.4%). Compared with the control group, six protein spots were significantly different in CGRP-induced group and expression of the 6 differently proteins was downregulated. Mass spectrometry analysis identified 5 proteins, including ribosome binding protein p180 which is related to new synthetic protein's translocation and calcium-binding protein (HRNR Hornerin) that can affect intracellular calcium concentration to regulate cell activity. These changes of proteome, including several groups of proteins that influence calcium ion concentration and new protein translocation, suggested that CGRP may regulate the cells by the second messengers and cytokines. This study contributes to a better understanding of the molecular mechanisms in CGRP-induced MG-63 cells.