The T-cell receptor V delta genes predominantly used by human peripheral gamma/delta+ T lymphocytes are not rearranged in CD3- natural killer cells.
Hum Immunol
; 26(2): 75-83, 1989 Oct.
Article
em En
| MEDLINE
| ID: mdl-2531131
ABSTRACT
We have analyzed, in 19 CD3- natural killer cell clones, the genomic organization of the T-cell receptor delta locus with two distinct V delta probes, V delta 1 and V delta 2. These two V delta genes code for surface proteins expressed in more than 90% of peripheral blood T-cell receptor gamma/delta+ lymphocytes, as shown by double color immunofluorescence analysis with anti-TCR delta 1, anti-BB3, and anti-delta TCS1 monoclonal antibodies. The V delta 1 and V delta 2 genes were found to be in germline position in all these clones, which are distinct phenotypically and represent a variety of the corresponding peripheral natural killer cell populations. We also studied in these cloned cell lines the transcriptional activity of the T-cell receptor delta locus with a C delta probe short transcripts (1.7 and 0.8 kb) were found exclusively. These experiments further suggest that CD3- natural killer peripheral cells are likely to constitute a unique lineage distinct from T lymphocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Matadoras Naturais
/
Receptores de Antígenos de Linfócitos T
/
Linfócitos T
/
Rearranjo Gênico
/
Antígenos de Diferenciação de Linfócitos T
Limite:
Humans
Idioma:
En
Revista:
Hum Immunol
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
França