Clofibrate increases long-chain fatty acid oxidation by neonatal pigs.
J Nutr
; 144(11): 1688-93, 2014 Nov.
Article
em En
| MEDLINE
| ID: mdl-25332467
ABSTRACT
BACKGROUND:
Utilization of energy-dense lipid fuels is critical to the rapid development and growth of neonates.OBJECTIVE:
To increase efficiency of milk fat utilization by newborn pigs, the effect of clofibrate on in vivo and in vitro long-chain fatty acid (LCFA) oxidation was evaluated.METHODS:
Newborn male pigs were administered 5 mL of vehicle (2% Tween 80) with or without clofibrate (75 mg/kg body weight) once daily via i.g. gavage for 4 d. Total LCFA oxidative capacity was measured in respiration chambers after gastric infusion (n = 5 per treatment) with isoenergetic amounts of [1-(14)C]triglycerides (TGs), either oleic acid (181n-9) TG [3.02 mmol/kg body weight (BW)(0.75)] or erucic acid (221n-9) TG (2.46 mmol/kg BW(0.75)). Total expired (14)CO2 was collected and quantified at 20-min intervals over 24 h. Hepatic in vitro LCFA oxidation was determined simultaneously using [1-(14)C]oleic acid and erucic acid substrates.RESULTS:
The in vivo 24-h accumulative [1-(14)C]TG oxidation (percentage of energy intake/kg BW(0.75)) tended to increase with clofibrate supplementation (P = 0.10), although there was no difference in the peak or mean utilization rate. The maximal extent of oleic acid TG oxidation was 1.6-fold that of erucic acid TG (P < 0.006). Hepatic in vitro LCFA oxidation increased 61% with clofibrate (P < 0.0008). The increase in mitochondria was 4-fold greater than in peroxisomes. The relative abundance of mRNA increased 2- to 3-fold for hepatic peroxisome proliferator-activated receptor α and its target genes (fatty acyl-coenzyme A oxidase and carnitine palmitoyltransferase) in the pigs that were administered clofibrate (P < 0.04).CONCLUSION:
Clofibrate may improve in vivo LCFA oxidative utilization in neonatal pigs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Clofibrato
/
Ácidos Graxos
/
Anticolesterolemiantes
Limite:
Animals
Idioma:
En
Revista:
J Nutr
Ano de publicação:
2014
Tipo de documento:
Article