Dynamic nuclear polarization-enhanced NMR on aligned lipid bilayers at ambient temperature.
J Am Chem Soc
; 136(44): 15533-6, 2014 Nov 05.
Article
em En
| MEDLINE
| ID: mdl-25333422
ABSTRACT
Dynamic nuclear polarization (DNP)-enhanced solid-state NMR spectroscopy has been shown to hold great potential for functional studies of membrane proteins at low temperatures due to its great sensitivity improvement. There are, however, numerous applications for which experiments at ambient temperature are desirable and which would also benefit from DNP signal enhancement. Here, we demonstrate as a proof of concept that a significant signal increase for lipid bilayers under room-temperature conditions can be achieved by utilizing the Overhauser effect. Experiments were carried out on aligned bilayers at 400 MHz/263 GHz using a stripline structure combined with a Fabry-Perot microwave resonator. A signal enhancement of protons of up to -10 was observed. Our results demonstrate that Overhauser DNP at high field provides efficient polarization transfer within insoluble samples, which is driven by fast local molecular fluctuations. Furthermore, our experimental setup offers an attractive option for DNP-enhanced solid-state NMR on ordered membranes and provides a general perspective toward DNP at ambient temperatures.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Temperatura
/
Espectroscopia de Ressonância Magnética
/
Bicamadas Lipídicas
Idioma:
En
Revista:
J Am Chem Soc
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Alemanha