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Congo red modulates ACh-induced Ca(2+) oscillations in single pancreatic acinar cells of mice.
Huang, Ze-bing; Wang, Hai-yan; Sun, Na-na; Wang, Jing-ke; Zhao, Meng-qin; Shen, Jian-xin; Gao, Ming; Hammer, Ronald P; Fan, Xue-gong; Wu, Jie.
Afiliação
  • Huang ZB; 1] Department of Infectious Diseases, Xiangya Hospital, Central South University, and Key Laboratory of Viral Hepatitis, Changsha 410008, China [2] Divisions of Neurology and Neurobiology, Barrow Neurological Institute, St Joseph's Hospital and Medical Center, Phoenix, AZ 8501, USA [3] Departments o
  • Wang HY; Department of Physiology, Shantou University of Medical College, Shantou 210854, China.
  • Sun NN; Department of Physiology, Shantou University of Medical College, Shantou 210854, China.
  • Wang JK; Department of Physiology, Shantou University of Medical College, Shantou 210854, China.
  • Zhao MQ; Department of Physiology, Shantou University of Medical College, Shantou 210854, China.
  • Shen JX; Department of Physiology, Shantou University of Medical College, Shantou 210854, China.
  • Gao M; Divisions of Neurology and Neurobiology, Barrow Neurological Institute, St Joseph's Hospital and Medical Center, Phoenix, AZ 8501, USA.
  • Hammer RP; Departments of Basic Medical Sciences, Pharmacology and Psychiatry, University of Arizona College of Medicine, Phoenix, AZ 85004, USA.
  • Fan XG; Department of Infectious Diseases, Xiangya Hospital, Central South University, and Key Laboratory of Viral Hepatitis, Changsha 410008, China.
  • Wu J; 1] Department of Infectious Diseases, Xiangya Hospital, Central South University, and Key Laboratory of Viral Hepatitis, Changsha 410008, China [2] Divisions of Neurology and Neurobiology, Barrow Neurological Institute, St Joseph's Hospital and Medical Center, Phoenix, AZ 8501, USA [3] Department of
Acta Pharmacol Sin ; 35(12): 1514-20, 2014 Dec.
Article em En | MEDLINE | ID: mdl-25345744
AIM: Congo red, a secondary diazo dye, is usually used as an indicator for the presence of amyloid fibrils. Recent studies show that congo red exerts neuroprotective effects in a variety of models of neurodegenerative diseases. However, its pharmacological profile remains unknown. In this study, we investigated the effects of congo red on ACh-induced Ca(2+) oscillations in mouse pancreatic acinar cells in vitro. METHODS: Acutely dissociated pancreatic acinar cells of mice were prepared. A U-tube drug application system was used to deliver drugs into the bath. Intracellular Ca(2+) oscillations were monitored by whole-cell recording of Ca(2+)-activated Cl(-) currents and by using confocal Ca(2+) imaging. For intracellular drug application, the drug was added in pipette solution and diffused into cell after the whole-cell configuration was established. RESULTS: Bath application of ACh (10 nmol/L) induced typical Ca(2+) oscillations in dissociated pancreatic acinar cells. Addition of congo red (1, 10, 100 µmol/L) dose-dependently enhanced Ach-induced Ca(2+) oscillations, but congo red alone did not induce any detectable response. Furthermore, this enhancement depended on the concentrations of ACh: congo red markedly enhanced the Ca(2+) oscillations induced by ACh (10-30 nmol/L), but did not alter the Ca(2+) oscillations induced by ACh (100-10000 nmol/L). Congo red also enhanced the Ca(2+) oscillations induced by bath application of IP3 (30 µmol/L). Intracellular application of congo red failed to alter ACh-induced Ca(2+) oscillations. CONCLUSION: Congo red significantly modulates intracellular Ca(2+) signaling in pancreatic acinar cells, and this pharmacological effect should be fully considered when developing congo red as a novel therapeutic drug.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Acetilcolina / Vermelho Congo / Sinalização do Cálcio / Pâncreas Exócrino Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Acta Pharmacol Sin Assunto da revista: FARMACOLOGIA Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Acetilcolina / Vermelho Congo / Sinalização do Cálcio / Pâncreas Exócrino Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Acta Pharmacol Sin Assunto da revista: FARMACOLOGIA Ano de publicação: 2014 Tipo de documento: Article