[TXNDC5 mediates serum starvation-induced proliferation inhibition of HeLa cell].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
; 36(5): 470-6, 2014 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-25360642
ABSTRACT
OBJECTIVE:
To investigate the role of TXNDC5 in serum starvation-induced proliferation inhibition of HeLa cell.METHODS:
TXNDC5 was either over-expressed or knocked down by small interfering RNA (siRNA) in HeLa cells which were then cultured in conventional medium or serum starvation medium. The protein level of TXNDC5 was evaluated by Western blot analysis. The mRNA level of TXNDC5 was measured by quantitative real-time PCR. Cell growth rate was determined by cell proliferation assay kit (MTS method). Cell cycle distribution and apoptosis were detected by flow cytometry.RESULTS:
Serum starvation mildly reduced the mRNA level of TXNDC5 (P<0.05), but dramatically increased the protein level of TXNDC5 in HeLa cells. The stability of TXNDC5 mRNA remained unchanged. Cycloheximide abolished the serum starvation-induced up-regulation of TXNDC5 protein. Over-expression of TXNDC5 had no effect on cell proliferation. However, suppression of TXNDC5 attenuated the proliferation inhibition of HeLa cell induced by serum starvation (P<0.05), increased the proportion of cells in S phase (P<0.05), but had no effect on cell apoptosis.CONCLUSION:
TXNDC5 mediates serum starvation-induced proliferation inhibition of HeLa cell.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Isomerases de Dissulfetos de Proteínas
/
Proliferação de Células
Limite:
Humans
Idioma:
Zh
Revista:
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China