Endoplasmic reticulum stress-mediated aldosterone-induced apoptosis in vascular endothelial cells.
J Huazhong Univ Sci Technolog Med Sci
; 34(6): 821-824, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25480576
ABSTRACT
The aim of this study was to examine the effects of endoplasmic reticulum (ER) stress on aldosterone (Aldo)-induced apoptosis of endothelial cells. Glucose-regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP, a hallmark of ER-associated apoptosis) were used to evaluate ER stress. Western blotting and real-time PCR were used to analyze indicators of ER molecule. Apoptosis was detected by annexin V/propidium iodide staining and flow cytometry. Human umbilical vein endothelial cells (HUVECs) were stimulated with different concentrations of Aldo for different durations. Aldo promoted apoptosis of HUVECs and induced ER stress, as evidenced by increased expression of GRP78 and CHOP. siRNA knockdown of CHOP attenuated Aldo-mediated apoptosis. These results indicate that ER stress may be involved in Aldo-induced apoptosis of HUVECs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Aldosterona
/
Células Endoteliais da Veia Umbilical Humana
/
Estresse do Retículo Endoplasmático
Limite:
Humans
Idioma:
En
Revista:
J Huazhong Univ Sci Technolog Med Sci
Assunto da revista:
MEDICINA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China