Antimicrobial activity of Chlorhexidine, Peracetic acid and Sodium hypochlorite/etidronate irrigant solutions against Enterococcus faecalis biofilms.
Int Endod J
; 48(12): 1188-93, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-25515403
ABSTRACT
AIM:
To evaluate the antimicrobial effect of 2.5% sodium hypochlorite alone (NaOCl) and associated with 9% HEBP (NaOCl/HEBP), 2% peracetic acid (PAA) and 2% chlorhexidine (CHX), on the viability of Enterococcus faecalis biofilms attached to dentine.METHODOLOGY:
Biofilms of E. faecalis were grown on the surface of dentine blocks for 5 days and then exposed to the irrigating solutions for 3 min. Distilled water was used as the control. The total biovolume and the percentage of dead cells of the infected dentine were measured by means of confocal microscopy and the live/dead technique. Nonparametric tests were used to determine statistical differences (P < 0.05).RESULTS:
NaOCl and the NaOCl/HEBP mixture were associated with a significantly greater percentage of dead cells, followed by PAA (P < 0.05). No significant antimicrobial effect of CHX was observed in comparison with the control group. Total biovolume decreased significantly in NaOCl, NaOCl/HEBP and PAA solutions in comparison with the CHX and control groups.CONCLUSIONS:
NaOCl alone or associated with HEBP were the most effective irrigant solutions in dissolving and killing E. faecalis biofilms.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Peracético
/
Irrigantes do Canal Radicular
/
Hipoclorito de Sódio
/
Clorexidina
/
Ácido Etidrônico
/
Enterococcus faecalis
/
Biofilmes
/
Dentina
/
Conservadores da Densidade Óssea
/
Anti-Infecciosos
Limite:
Humans
Idioma:
En
Revista:
Int Endod J
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Espanha