Biomarkers of brevetoxin exposure and composite toxin levels in hard clam (Mercenaria sp.) exposed to Karenia brevis blooms.
Toxicon
; 96: 82-8, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25620222
Brevetoxins in clams (Mercenaria sp.) exposed to recurring blooms of Karenia brevis in Sarasota Bay, FL, were studied over a three-year period. Brevetoxin profiles in toxic clams were generated by ELISA and LC-MS. Several brevetoxin metabolites, as identified by LC-MS, were major contributors to the composite brevetoxin response of ELISA. These were S-desoxyBTX-B2 (m/z 1018), BTX-B2 (m/z 1034), BTX-B5 (m/z 911), open A-ring BTX-B5 (m/z 929), and BTX-B1 (m/z 1018). Summed values of these metabolites were highly correlated (R(2) = 0.9) with composite B-type brevetoxin measurements by ELISA. S-desoxyBTX-B2, BTX-B2, and BTX-B1 were the most persistent and detectable in shellfish for several months after dissipation of blooms. These metabolites were selected as LC-MS biomarkers of brevetoxin exposure and reflective of composite B-type brevetoxins in hard clam. ELISA and LC-MS values were moderately correlated with toxicity of the shellfish by mouse bioassay. ELISA and LC-MS methods offer rapid screening and confirmatory determination of brevetoxins, respectively, as well as toxicity assessment in clams exposed to K. brevis blooms.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dinoflagellida
/
Biomarcadores
/
Bivalves
/
Oxocinas
/
Exposição Ambiental
/
Proliferação Nociva de Algas
/
Toxinas Marinhas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
País/Região como assunto:
America do norte
Idioma:
En
Revista:
Toxicon
Ano de publicação:
2015
Tipo de documento:
Article