Cell cycle control of spindle pole body duplication and splitting by Sfi1 and Cdc31 in fission yeast.
J Cell Sci
; 128(8): 1481-93, 2015 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-25736294
ABSTRACT
Spindle pole biogenesis and segregation are tightly coordinated to produce a bipolar mitotic spindle. In yeasts, the spindle pole body (SPB) half-bridge composed of Sfi1 and Cdc31 duplicates to promote the biogenesis of a second SPB. Sfi1 accumulates at the half-bridge in two phases in Schizosaccharomyces pombe, from anaphase to early septation and throughout G2 phase. We found that the function of Sfi1-Cdc31 in SPB duplication is accomplished before septation ends and G2 accumulation starts. Thus, Sfi1 early accumulation at mitotic exit might correspond to half-bridge duplication. We further show that Cdc31 phosphorylation on serine 15 in a Cdk1 (encoded by cdc2) consensus site is required for the dissociation of a significant pool of Sfi1 from the bridge and timely segregation of SPBs at mitotic onset. This suggests that the Cdc31 N-terminus modulates the stability of Sfi1-Cdc31 arrays in fission yeast, and impacts on the timing of establishment of spindle bipolarity.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
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Proteínas de Ligação a Calmodulina
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Proteínas de Ligação ao Cálcio
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Proteínas de Ciclo Celular
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Proteínas de Schizosaccharomyces pombe
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Pontos de Checagem do Ciclo Celular
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Corpos Polares do Fuso
Idioma:
En
Revista:
J Cell Sci
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
França