High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus.
Theor Appl Genet
; 128(6): 1039-47, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-25754422
ABSTRACT
KEY MESSAGE We characterise the distribution of crossover and non-crossover recombination in Brassica napus and Cicer arietinum using a low-coverage genotyping by sequencing pipeline SkimGBS. The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Troca Genética
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Brassica napus
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Cicer
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Técnicas de Genotipagem
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Conversão Gênica
Idioma:
En
Revista:
Theor Appl Genet
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Austrália