Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis.
Biomed Opt Express
; 6(4): 1512-9, 2015 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-25909032
ABSTRACT
We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200µm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.
(060.2310) Fiber optics; (110.2760) Gradient-index lenses; (170.0170) Medical optics and biotechnology; (170.3880) Medical and biological imaging; (170.4520) Optical confinement and manipulation; (180.0180) Microscopy; (180.2520) Fluorescence microscopy; (350.4855) Optical tweezers or optical manipulation
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Biomed Opt Express
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Reino Unido