Rap1 Spatially Controls ArhGAP29 To Inhibit Rho Signaling during Endothelial Barrier Regulation.
Mol Cell Biol
; 35(14): 2495-502, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-25963656
ABSTRACT
The small GTPase Rap1 controls the actin cytoskeleton by regulating Rho GTPase signaling. We recently established that the Rap1 effectors Radil and Rasip1, together with the Rho GTPase activating protein ArhGAP29, mediate Rap1-induced inhibition of Rho signaling in the processes of epithelial cell spreading and endothelial barrier function. Here, we show that Rap1 induces the independent translocations of Rasip1 and a Radil-ArhGAP29 complex to the plasma membrane. This results in the formation of a multimeric protein complex required for Rap1-induced inhibition of Rho signaling and increased endothelial barrier function. Together with the previously reported spatiotemporal control of the Rap guanine nucleotide exchange factor Epac1, these findings elucidate a signaling pathway for spatiotemporal control of Rho signaling that operates by successive protein translocations to and complex formation at the plasma membrane.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
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Proteínas rap1 de Ligação ao GTP
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Proteínas rho de Ligação ao GTP
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Proteínas Ativadoras de GTPase
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Células Endoteliais da Veia Umbilical Humana
Limite:
Humans
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Holanda