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The non-proliferative nature of ascidian folliculogenesis as a model of highly ordered cellular topology distinct from proliferative epithelia.
Azzag, Karim; Chelin, Yoann; Rousset, François; Le Goff, Emilie; Martinand-Mari, Camille; Martinez, Anne-Marie; Maurin, Bernard; Daujat-Chavanieu, Martine; Godefroy, Nelly; Averseng, Julien; Mangeat, Paul; Baghdiguian, Stephen.
Afiliação
  • Azzag K; Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, Cedex 5, France; Institut des Sciences de l'Evolution (ISE-M), CNRS, Montpellier, France.
  • Chelin Y; Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, Cedex 5, France; Laboratoire de Mécanique et Génie Civil (LMGC), CNRS, Montpellier, France.
  • Rousset F; Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, Cedex 5, France; Institut des Sciences de l'Evolution (ISE-M), CNRS, Montpellier, France.
  • Le Goff E; Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, Cedex 5, France; Institut des Sciences de l'Evolution (ISE-M), CNRS, Montpellier, France.
  • Martinand-Mari C; Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, Cedex 5, France; Institut des Sciences de l'Evolution (ISE-M), CNRS, Montpellier, France.
  • Martinez AM; Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, Cedex 5, France; Institut de Génétique Humaine (IGH), CNRS, Montpellier, France.
  • Maurin B; Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, Cedex 5, France; Laboratoire de Mécanique et Génie Civil (LMGC), CNRS, Montpellier, France.
  • Daujat-Chavanieu M; Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, Cedex 5, France; INSERM U1040, Montpellier, France; CHU Montpellier, Institut de Biothérapie, Montpellier, France.
  • Godefroy N; Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, Cedex 5, France; Institut des Sciences de l'Evolution (ISE-M), CNRS, Montpellier, France.
  • Averseng J; Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, Cedex 5, France; Laboratoire de Mécanique et Génie Civil (LMGC), CNRS, Montpellier, France.
  • Mangeat P; Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, Cedex 5, France; Centre de Recherche de Biochimie Macromoléculaire (CRBM), CNRS, Montpellier, France.
  • Baghdiguian S; Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, Cedex 5, France; Institut des Sciences de l'Evolution (ISE-M), CNRS, Montpellier, France.
PLoS One ; 10(5): e0126341, 2015.
Article em En | MEDLINE | ID: mdl-26000769
ABSTRACT
Previous studies have addressed why and how mono-stratified epithelia adopt a polygonal topology. One major additional, and yet unanswered question is how the frequency of different cell shapes is achieved and whether the same distribution applies between non-proliferative and proliferative epithelia. We compared different proliferative and non-proliferative epithelia from a range of organisms as well as Drosophila melanogaster mutants, deficient for apoptosis or hyperproliferative. We show that the distribution of cell shapes in non-proliferative epithelia (follicular cells of five species of tunicates) is distinctly, and more stringently organized than proliferative ones (cultured epithelial cells and Drosophila melanogaster imaginal discs). The discrepancy is not supported by geometrical constraints (spherical versus flat monolayers), number of cells, or apoptosis events. We have developed a theoretical model of epithelial morphogenesis, based on the physics of divided media, that takes into account biological parameters such as cell-cell contact adhesions and tensions, cell and tissue growth, and which reproduces the effects of proliferation by increasing the topological heterogeneity observed experimentally. We therefore present a model for the morphogenesis of epithelia where, in a proliferative context, an extended distribution of cell shapes (range of 4 to 10 neighbors per cell) contrasts with the narrower range of 5-7 neighbors per cell that characterizes non proliferative epithelia.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proliferação de Células / Células Epiteliais / Epitélio / Morfogênese Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: França

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proliferação de Células / Células Epiteliais / Epitélio / Morfogênese Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: França