Relative Expression of Low Molecular Weight Protein, Tyrosine Phosphatase (Wzb Gene) of Herbaspirillum sp. GW103 Toward Arsenic Stress and Molecular Modeling.

ABSTRACT

This study investigated the expression rate and molecular modeling of Wzb gene, a low molecular weight protein tyrosine phosphatase, under As stress in Herbaspirillum sp. GW103. Expression of Wzb gene was quantified at transcriptional level through real-time quantitative PCR. The results showed up- and down-regulations of Wzb gene in the presence of As (50 and 100 mg/L). The maximum Wzb transcript expression was 1.2-fold after 72 h exposure to 50 mg/L of As. However, the minimum expression was 0.1-fold after 48 h exposure to 100 mg/L of As. The Wzb protein sequence was retrieved from NCBI sequence database and was used for in silico analysis. 3D structure of Wzb gene was predicted by comparative modeling using modeler 9v9. Further, the model was validated for its quality by Ramachandran plot, ERRAT, Verify 3D, and SAVES server which revealed structure and quality of the Wzb gene model.