The content of docosahexaenoic acid in the maternal diet differentially affects the immune response in lactating dams and suckled offspring.
Eur J Nutr
; 55(7): 2255-64, 2016 Oct.
Article
em En
| MEDLINE
| ID: mdl-26358165
ABSTRACT
PURPOSE:
The objective of this study was to determine the effect of feeding a maternal diet supplemented with docosahexaenoic acid (DHA) while also containing adequate amounts of arachidonic acid on immune system development and function in suckled offspring and lactating rats.METHODS:
Sprague-Dawley dams were randomized to one of the two nutritionally adequate experimental diets 24-48 h prior to parturition control diet (N = 12, 0 % DHA) or high DHA diet (N = 8, 0.9 % DHA of total fatty acids). Diets were fed throughout the lactating/suckling period (21 days), and then, dams and pups were terminated, and immune cell phenotypes and cytokine production by mitogen- or ovalbumin-stimulated splenocytes were measured.RESULTS:
Feeding dams a high DHA diet resulted in a higher proportion of 183n-3, 225n-3 and 226n-3 found in pup's stomach content (breast milk; P < 0.01). Feeding the high DHA diet had no impact on growth parameters or the ex vivo cytokine production by mitogen-stimulated splenocytes in both dams and pups. There was a higher proportion of OX12+CD80+ cells and a lower production of TGF-ß by splenocytes after ovalbumin stimulation in pups from dams fed the DHA diet (both P < 0.05) while maintaining a similar IL-2 production. LPS-stimulated splenocytes from dams fed the high DHA diet produced more TNF-α versus control diet (P < 0.05).CONCLUSIONS:
Overall, our results suggest that DHA supplementation in the maternal diet does not change the immune response to mitogens but positively affects the activation of B cells as well as the response to a potential food antigen upon challenge in suckled offspring.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lactação
/
Ácidos Docosa-Hexaenoicos
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Fenômenos Fisiológicos da Nutrição Materna
/
Dieta
Limite:
Animals
Idioma:
En
Revista:
Eur J Nutr
Assunto da revista:
CIENCIAS DA NUTRICAO
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Canadá