Detection of cytoplasmic proteins from Helicobacter pylori in Colony Lift Immunoassay.

Rojas-Rengifo, Diana F; Jaramillo, Carlos A; Haas, Rainer; Jiménez-Soto, Luisa F

Rojas-Rengifo, Diana F; Jaramillo, Carlos A; Haas, Rainer; Jiménez-Soto, Luisa F.

Afiliação

Rojas-Rengifo DF; Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität, Pettenkoferstrasse 9a, D-80336 München, Germany.
Jaramillo CA; Molecular Diagnostics and Bioinformatics Laboratory, Biological Sciences Department, Los Andes University, Carrera 1 # 18A-10, Bogotá, Colombia.
Haas R; Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität, Pettenkoferstrasse 9a, D-80336 München, Germany.
Jiménez-Soto LF; Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität, Pettenkoferstrasse 9a, D-80336 München, Germany. Electronic address: jimenez@mvp.uni-muenchen.de.

J Microbiol Methods ; 119: 145-6, 2015 Dec.

Article em En | MEDLINE | ID: mdl-26456045

ABSTRACT

ABSTRACT

Use of the Colony Lift Immunoassay has been described for several Gram negative bacteria of medical interest. In all cases detection was limited to the use of antibodies against outer membrane proteins. Here we describe the adaptation of this method for detection of the cytoplasmic CagA toxin from Helicobacter pylori.

Assuntos

Proteínas de Bactérias/análise; Infecções por Helicobacter/microbiologia; Helicobacter pylori/metabolismo; Imunoensaio/métodos; Proteínas de Bactérias/genética; Proteínas de Bactérias/metabolismo; Citoplasma/química; Citoplasma/genética; Citoplasma/metabolismo; Helicobacter pylori/química; Helicobacter pylori/genética; Helicobacter pylori/isolamento & purificação; Humanos

Palavras-chave

CLI; Colony blot; Cytoplasmic proteins; Helicobacter pylori; Immunoassay

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Imunoensaio / Helicobacter pylori / Infecções por Helicobacter Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Revista: J Microbiol Methods Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Alemanha

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