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An Effective Immuno-PET Imaging Method to Monitor CD8-Dependent Responses to Immunotherapy.
Tavaré, Richard; Escuin-Ordinas, Helena; Mok, Stephen; McCracken, Melissa N; Zettlitz, Kirstin A; Salazar, Felix B; Witte, Owen N; Ribas, Antoni; Wu, Anna M.
Afiliação
  • Tavaré R; Crump Institute for Molecular Imaging, University of California Los Angeles, Los Angeles, California. Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California. rtavare@mednet.ucla.edu awu@mednet.ucla.edu.
  • Escuin-Ordinas H; Department of Medicine, Division of Hematology-Oncology, University of California Los Angeles, Los Angeles, California.
  • Mok S; Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California.
  • McCracken MN; Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California.
  • Zettlitz KA; Crump Institute for Molecular Imaging, University of California Los Angeles, Los Angeles, California. Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California.
  • Salazar FB; Crump Institute for Molecular Imaging, University of California Los Angeles, Los Angeles, California. Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California.
  • Witte ON; Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California. Howard Hughes Medical Institute, University of California Los Angeles, Los Angeles, California. Department of Microbiology, Immunology and Molecular Genetics, University of California Los
  • Ribas A; Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California. Department of Medicine, Division of Hematology-Oncology, University of California Los Angeles, Los Angeles, California. Jonsson Comprehensive Cancer Center, University of California Los A
  • Wu AM; Crump Institute for Molecular Imaging, University of California Los Angeles, Los Angeles, California. Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California. Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angele
Cancer Res ; 76(1): 73-82, 2016 Jan 01.
Article em En | MEDLINE | ID: mdl-26573799
ABSTRACT
The rapidly advancing field of cancer immunotherapy is currently limited by the scarcity of noninvasive and quantitative technologies capable of monitoring the presence and abundance of CD8(+) T cells and other immune cell subsets. In this study, we describe the generation of (89)Zr-desferrioxamine-labeled anti-CD8 cys-diabody ((89)Zr-malDFO-169 cDb) for noninvasive immuno-PET tracking of endogenous CD8(+) T cells. We demonstrate that anti-CD8 immuno-PET is a sensitive tool for detecting changes in systemic and tumor-infiltrating CD8 expression in preclinical syngeneic tumor immunotherapy models including antigen-specific adoptive T-cell transfer, agonistic antibody therapy (anti-CD137/4-1BB), and checkpoint blockade antibody therapy (anti-PD-L1). The ability of anti-CD8 immuno-PET to provide whole body information regarding therapy-induced alterations of this dynamic T-cell population provides new opportunities to evaluate antitumor immune responses of immunotherapies currently being evaluated in the clinic.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Radioisótopos / Zircônio / Imunoterapia Adotiva / Neoplasias do Colo / Linfócitos T CD8-Positivos / Compostos Radiofarmacêuticos / Tomografia por Emissão de Pósitrons Limite: Animals / Humans Idioma: En Revista: Cancer Res Ano de publicação: 2016 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Radioisótopos / Zircônio / Imunoterapia Adotiva / Neoplasias do Colo / Linfócitos T CD8-Positivos / Compostos Radiofarmacêuticos / Tomografia por Emissão de Pósitrons Limite: Animals / Humans Idioma: En Revista: Cancer Res Ano de publicação: 2016 Tipo de documento: Article