Evaluation and optimization of a commercial blocking ELISA for detecting antibodies to influenza A virus for research and surveillance of mallards.
J Virol Methods
; 228: 130-4, 2016 Feb.
Article
em En
| MEDLINE
| ID: mdl-26645952
ABSTRACT
The availability of a validated commercial assay is an asset for any wildlife investigation. However, commercial products are often developed for use in livestock and are not optimized for wildlife. Consequently, it is incumbent upon researchers and managers to apply commercial products appropriately to optimize program outcomes. We tested more than 800 serum samples from mallards for antibodies to influenza A virus with the IDEXX AI MultiS-Screen Ab test to evaluate assay performance. Applying the test per manufacturer's recommendations resulted in good performance with 84% sensitivity and 100% specificity. However, performance was improved to 98% sensitivity and 98% specificity by increasing the recommended cut-off. Using this alternative threshold for identifying positive and negative samples would greatly improve sample classification, especially for field samples collected months after infection when antibody titers have waned from the initial primary immune response. Furthermore, a threshold that balances sensitivity and specificity reduces estimation bias in seroprevalence estimates.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
/
Ensaio de Imunoadsorção Enzimática
/
Patos
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Monitoramento Epidemiológico
/
Influenza Aviária
/
Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
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Screening_studies
Limite:
Animals
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
2016
Tipo de documento:
Article