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Simultaneous achiral-chiral analysis of pharmaceutical compounds using two-dimensional reversed phase liquid chromatography-supercritical fluid chromatography.
Venkatramani, C J; Al-Sayah, Mohammad; Li, Guannan; Goel, Meenakshi; Girotti, James; Zang, Lisa; Wigman, Larry; Yehl, Peter; Chetwyn, Nik.
Afiliação
  • Venkatramani CJ; Small Molecule Analytical Chemistry & Quality Control, Genentech, Inc. So., San Francisco, CA 94080, United States. Electronic address: cvenkatramani@yahoo.com.
  • Al-Sayah M; Small Molecule Analytical Chemistry & Quality Control, Genentech, Inc. So., San Francisco, CA 94080, United States. Electronic address: alsayah.mohammad@gene.com.
  • Li G; University of Illinois at Chicago, Chicago, IL 60612, United States.
  • Goel M; Small Molecule Analytical Chemistry & Quality Control, Genentech, Inc. So., San Francisco, CA 94080, United States.
  • Girotti J; Small Molecule Analytical Chemistry & Quality Control, Genentech, Inc. So., San Francisco, CA 94080, United States.
  • Zang L; Agilent Technologies, Santa Clara, CA 95051, United States.
  • Wigman L; Small Molecule Analytical Chemistry & Quality Control, Genentech, Inc. So., San Francisco, CA 94080, United States.
  • Yehl P; Small Molecule Analytical Chemistry & Quality Control, Genentech, Inc. So., San Francisco, CA 94080, United States.
  • Chetwyn N; Small Molecule Analytical Chemistry & Quality Control, Genentech, Inc. So., San Francisco, CA 94080, United States.
Talanta ; 148: 548-55, 2016 Feb 01.
Article em En | MEDLINE | ID: mdl-26653484
ABSTRACT
A new interface was designed to enable the coupling of reversed phase liquid chromatography (RPLC) and supercritical fluid chromatography (SFC). This online two-dimensional chromatographic system utilizing RPLC in the first dimension and SFC in the second was developed to achieve simultaneous achiral and chiral analysis of pharmaceutical compounds. The interface consists of an eight-port, dual-position switching valve with small volume C-18 trapping columns. The peaks of interest eluting from the first RPLC dimension column were effectively focused as sharp concentration pulses on small volume C-18 trapping column/s and then injected onto the second dimension SFC column. The first dimension RPLC separation provides the achiral purity result, and the second dimension SFC separation provides the chiral purity result (enantiomeric excess). The results are quantitative enabling simultaneous achiral, chiral analysis of compounds. The interface design and proof of concept demonstration are presented. Additionally, comparative studies to conventional SFC and case studies of the applications of 2D LC-SFC in pharmaceutical analysis is presented.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Preparações Farmacêuticas / Cromatografia com Fluido Supercrítico / Cromatografia de Fase Reversa Idioma: En Revista: Talanta Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Preparações Farmacêuticas / Cromatografia com Fluido Supercrítico / Cromatografia de Fase Reversa Idioma: En Revista: Talanta Ano de publicação: 2016 Tipo de documento: Article