COMP-assisted collagen secretion--a novel intracellular function required for fibrosis.
J Cell Sci
; 129(4): 706-16, 2016 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-26746240
ABSTRACT
Cartilage oligomeric matrix protein (COMP) is an abundant component in the extracellular matrix (ECM) of load-bearing tissues such as tendons and cartilage. It provides adaptor functions by bridging different ECM structures. We have previously shown that COMP is also a constitutive component of healthy human skin and is strongly induced in fibrosis. It binds directly and with high affinity to collagen I and to collagen XII that decorates the surface of collagen I fibrils. We demonstrate here that lack of COMP-collagen interaction in the extracellular space leads to changes in collagen fibril morphology and density, resulting in altered skin biomechanical properties. Surprisingly, COMP also fulfills an important intracellular function in assisting efficient secretion of collagens, which were retained in the endoplasmic reticulum of COMP-null fibroblasts. Accordingly, COMP-null mice showed severely attenuated fibrotic responses in skin. Collagen secretion was fully restored by introducing wild-type COMP. Hence, our work unravels a new, non-structural and intracellular function of the ECM protein COMP in controlling collagen secretion.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Colágenos Fibrilares
/
Proteína de Matriz Oligomérica de Cartilagem
Limite:
Animals
Idioma:
En
Revista:
J Cell Sci
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Alemanha