Genetic and infective diversity of the liver fluke Fasciola hepatica (Trematoda: Digenea) from Cuba.
J Helminthol
; 90(6): 719-725, 2016 Nov.
Article
em En
| MEDLINE
| ID: mdl-26765141
ABSTRACT
In this study we present the first approach to exploration of the genetic diversity of Cuban Fasciola hepatica populations using microsatellite markers, coupled with observed prevalence in slaughterhouses. Nine populations of flukes recovered from cows and buffalos were studied in the central-western region of Cuba. The observed infection rates of definitive hosts (bovines) were 70-100% in most cases. An important amount of polymorphism was found in the four loci explored. However, no apparent genetic differences were found between populations from different provinces or bovine species. The absence of deviations from Hardy-Weinberg equilibrium suggests a high rate of cross-fertilization between F. hepatica individuals. This result was confirmed when all multilocus genotypes were tested for clonal reproduction and only four individuals differed statistically (P sex< 0.05). High values of expected heterozygosity coupled with highly probable mixing among strains make the metapopulation genetically diversified but similar in terms of certain alleles (low F ST values). These results suggest a close relationship between parasite diversity and cattle management in Cuba. Our findings should be taken into consideration by veterinary authorities to help mitigate fasciolosis transmission.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Variação Genética
/
Doenças dos Bovinos
/
Fasciola hepatica
/
Fasciolíase
Tipo de estudo:
Prevalence_studies
/
Risk_factors_studies
Limite:
Animals
País/Região como assunto:
Caribe
/
Cuba
Idioma:
En
Revista:
J Helminthol
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
França