Peroxisome proliferator-activated receptor ß/δ (PPARß/δ) activates promyogenic signaling pathways, thereby promoting myoblast differentiation.
Biochem Biophys Res Commun
; 470(1): 157-162, 2016 Jan 29.
Article
em En
| MEDLINE
| ID: mdl-26768366
ABSTRACT
Peroxisome proliferator-activated receptor ß/δ (PPARß/δ) regulates postnatal myogenesis by alleviating myostatin activity, but the molecular mechanisms by which it regulates myogenesis are not fully understood. In this study, we investigate molecular mechanisms of PPARß/δ in myoblast differentiation. C2C12 myoblasts treated with a PPARß/δ agonist, GW0742 exhibit enhanced myotube formation and muscle-specific gene expression. GW0742 treatment dramatically activates promyogenic kinases, p38MAPK and Akt, in a dose-dependent manner. GW0742-stimulated myoblast differentiation is mediated by p38MAPK and Akt, since it failed to restore myoblast differentiation repressed by inhibition of p38MAPK and Akt. In addition, GW0742 treatment enhances MyoD-reporter activities. Consistently, overexpression of PPARß/δ enhances myoblast differentiation accompanied by elevated activation of p38MAPK and Akt. Collectively, these results suggest that PPARß/δ enhances myoblast differentiation through activation of promyogenic signaling pathways.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fibras Musculares Esqueléticas
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Mioblastos
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PPAR beta
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PPAR gama
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2016
Tipo de documento:
Article