Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles.
Analyst
; 141(13): 3982-4, 2016 Jun 20.
Article
em En
| MEDLINE
| ID: mdl-26811852
ABSTRACT
Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Immobilization of the protein biosensor in the nanoparticle was achieved through specific interaction between the hexa-histidine tag of the protein and a calcium-silicate complex of the silica matrix. Encapsulation of the biosensors preserved the affinity for the respective sugar. Compared to the free biosensors, encapsulation had a stabilizing effect on the biosensor towards chemical and thermal denaturation. The demonstrated immobilization strategy for specific sensing proteins paves the way towards the development of protein-inorganic nanostructures for application in metabolite analyses.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Dióxido de Silício
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Transferência Ressonante de Energia de Fluorescência
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Nanopartículas
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Glucose
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Maltose
Idioma:
En
Revista:
Analyst
Ano de publicação:
2016
Tipo de documento:
Article