Review of lignocellulolytic enzyme activity analyses and scale-down to microplate-based assays.
Talanta
; 150: 629-37, 2016 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-26838452
ABSTRACT
With the increasing use of enzymes in environmental applications, there is a need for analytical methods adapted to large factorial experiments. Existing reference methods are chemical and labor intensive and unsuitable to analyze in parallel a large number of samples. Based on an extensive literature review and on experimental results, this work compares reference and microplate adapted methods to define the most adequate filter paper, carboxymethylcellulase, ß-glucosidase and xylanase activity tests. In the adapted methods, the total reaction volume was reduced from 2.2-24.5 mL to 0.21-0.24 mL. Statistical analysis of the activities measured on enzyme mixtures by applying the 96-well plate reduced methods showed that they were not significantly different to the activities obtained with reference tests.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bioensaio
/
Celulase
/
Técnicas de Química Analítica
/
Beta-Glucosidase
/
Endo-1,4-beta-Xilanases
/
Filtros Microporos
Idioma:
En
Revista:
Talanta
Ano de publicação:
2016
Tipo de documento:
Article